1.Genomics
2.Transcriptomics
3.Epigenetics
4.Proteomics
5.Metabolomics
Human Whole Genome Sequencing
Service Specifications
| Platform | Library | Sequencing Depth |
|---|---|---|
|
Illumina NovaSeq MGI DNBSEQ-T7 |
PE150 | ≥30 X |
| Nanopore PromethION 48 | 8 kb | ≥30 X |
| PacBio Revio | 15 kb - Hifi | ≥10 X |
Sample Requirements
| Platform | Concentration (ng/μL) | Total amount (μg) | OD260/280 | OD260/230 |
|---|---|---|---|---|
| Illumina/MGI PCR-free | ≥1 | ≥0.03 | - | Limited degradation and protein or RNA contamination |
| ≥40 | ≥0.5 | - | ||
| Nanopore | ≥40 | ≥4 | 1.7-2.2 | |
| PacBio | ≥50 | ≥10 | 1.7-2.2 |
Human Whole Exome Sequencing
Service Specifications
| Exon Capture Strategy | Sequencing Strategy | Recommended Data Output |
|---|---|---|
|
Sure Select Human All Exon v6 (Agilent) or xGen Exome Hybridization Panel v2 (IDT) |
Illumina NovaSeq PE150 |
5 - 10 Gb For mendelian disorders/rare diseases: > 50x For tumor samples: ≥ 100x |
Sample Requirements
| Sample Type | Amount (Qubit®) | Concentration | Volume | Purity (NanoDrop™) |
|---|---|---|---|---|
| Genomic DNA | ≥ 50 ng | ≥ 6 ng/μL | ≥ 15 μL |
OD260/280 = 1.8–2.0 no degradation, no contamination |
Plant/Animal Whole Genome Sequencing
Service Specifications
| Variants to be identified | Sequencing strategy | Recommended depth |
|---|---|---|
| SNP and InDel | Illumina NovaSeq PE150 or MGI T7 |
10x |
| SV and CNV (less accurate) | 30x | |
| SV and CNV (more accurate) | Nanopore Prom P48 | 20x |
| SNPs, Indels, SV and CNV | PacBio Revio | 10x |
Sample Requirements
| Tissue or extracted nucleic acids | Illumina/MGI | Nanopore | PacBio | ||
|---|---|---|---|---|---|
| Animal Viscera | 0.5 - 1 g | ≥ 3.5 g | ≥ 3.5 g | ||
| Animal Muscle | ≥ 5 g | ≥ 5 g | |||
| Mammalian Blood | 1.5 mL | ≥ 0.5 mL | ≥ 5 mL | ||
| Poultry/Fish Blood | ≥ 0.1 mL | ≥ 0.5 mL | |||
| Plant- Fresh Leaf | 1 - 2 g | ≥ 2 g | ≥ 5 g | ||
| Cultured Cells | ≥ 1×107 | ≥ 1×108 | |||
| Insect soft tissue/Individual | 0.5 - 1 g | ≥ 1 g | ≥ 3 g | ||
| Extracted DNA |
Concentration: ≥ 1 ng/μL Amount: ≥ 30 ng Limited or no degradation or contamination |
Concentration / Amount OD260/280 OD260/230 Limited or no degradation or contamination |
≥ 40 ng/ µL 4 µg/flow cell/sample 1.7 - 2.2 ≥ 1.5 |
Concentration / Amount OD260/280 OD260/230 Limited or no degradation or contamination |
≥ 50 ng/μL 10 μg/flow cell/sample 1.7 - 2.2 1.8 - 2.5 |
|
PCR-free Library Preparation: Concentration ≥ 40 ng/μL Amount ≥ 500 ng |
|||||
Plant/Animal De Novo Sequencing
Service Specification
| Genome survey | Genome assembly | Chromosome-level | Genome Annotation |
|---|---|---|---|
| 50X Illumina NovaSeq PE150 |
30X PacBio CCS HiFi reads |
100X Hi-C |
RNA-seq Illumina PE150 10 Gb + (optional) Full length RNA-seq PacBio 40 Gb or Nanopore 12 Gb |
Sample Requirements
| Tissue or extracted nucleic acids | Genome Survey | Genome Assembly with PacBio | Hi-C Assembly |
|---|---|---|---|
| Animal Viscera | 0.5–1 g | ≥ 3.5 g | ≥ 2 g |
| Animal Muscle | ≥ 5 g | ||
| Mammalian Blood | 1.5 mL | ≥ 5 mL | ≥ 2 mL |
| Poultry/Fish Blood | ≥ 0.5 mL | ||
| Plant - Fresh Leaf | 1–2 g | ≥ 5 g | ≥ 4 g |
| Cultured Cells | ≥ 1×108 | ≥ 1×107 | |
| Insect | 0.5–1 g | ≥ 3 g | ≥ 2 g |
| Extracted DNA |
Concentration: ≥ 1 ng/μL Amount: ≥ 30 ng Limited or no degradation or contamination |
Concentration: ≥ 50 ng/μL Amount: 10 μg/flow cell/sample OD260/280 = 1.7–2.2 OD260/230 = 1.8–2.5 Limited or no degradation or contamination |
– |
Bacterial and Fungal Whole Genome Re-Sequencing
Service Specifications
| Sequencing platform | Sequencing Strategy | Data recommended | Quality control |
|---|---|---|---|
| Illumina NovaSeq | PE150 | Depth of 100x | Q30 ≥ 85% |
Sample Requirement
| Concentration (ng/μL) | Total amount (ng) | Volume (μL) |
|---|---|---|
| ≥1 | ≥60 | ≥20 |
De Novo Bacterial Genome Assembly
Service Specifications
| Service | Sequencing Strategy |
|---|---|
| Draft Genome | Illumina PE150 100x |
| 0 Gap Genome | Nanopore 100x + Illumina PE150 100x or Pacbio HiFi 30x + Illumina PE150 100x (optional) |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Volume (μL) | OD260/280 | OD260/230 | |
|---|---|---|---|---|---|
| PacBio | ≥ 20 | ≥ 1.2 | ≥ 20 | 1.7-2.2 | ≥ 1.0 |
| Nanopore | ≥ 40 | ≥ 2 | ≥ 20 | 1.7-2.2 | 1.0-3.0 |
| Illumina | ≥ 1 | ≥ 0.06 | ≥ 20 | - | - |
De Novo Fungal Genome Assembly
Service Specifications
| Service | Sequencing Strategy | Quality Control |
|---|---|---|
| Draft Genome | Illumina PE150 | Q30 ≥ 85% |
| Fine Genome | Nanopore 50x + Illumina PE150 50x Assembly: PacBio HiFi 30x or Nanopore 100x + Illumina PE150 50x or Hi-C Assembly 100x | contig N50 ≥ 1 Mb; contig N50 ≥ 500kb (fungi) |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Volume (μL) | OD260/280 | OD260/230 | |
|---|---|---|---|---|---|
| PacBio | ≥ 20 | ≥ 1.2 | ≥ 20 | 1.7-2.2 | ≥ 1.0 |
| Nanopore | ≥ 40 | ≥ 2 | ≥ 20 | 1.7-2.2 | 1.0-3.0 |
| Illumina | ≥ 1 | ≥ 0.06 | ≥ 20 | - | - |
16S/18S/ITS Amplicon Sequencing - PacBio
Service Specifications
| Library | Sequencing Strategy | Data Recommended |
|---|---|---|
| Amplicon | PacBio Revio | 10/30/50 K tags (CCS) |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Volume (μL) |
|---|---|---|
| ≥ 5 | ≥ 0.3 | ≥ 20 |
16S/18S/ITS Amplicon Sequencing – Illumina NovaSeq
Service Specifications
| Library | Sequencing Strategy | Data recommended |
|---|---|---|
| Amplicon | Illumina PE250 | 50/100/300K tags (Read Pairs) |
Sample Requirements
| Concentration (ng/μL) | Total amount (ng) | Volume (μL) |
|---|---|---|
| ≥ 1 | ≥ 200 | ≥ 20 |
Metagenomic Sequencing
Service Specifications
| Sequencing platform | Sequencing Strategy | Data recommended |
|---|---|---|
| Illumina NovaSeq | PE150 | 6 Gb |
| Nanopore P48 | 10 kb | 6 / 10 Gb |
| PacBio Revio | 8 kb | 10 / 20 Gb |
Sample Requirements
| Library | Concentration (ng/μL) | Total amount (μg) | Volume (μL) |
|---|---|---|---|
| Illumina PE150 Library | ≥ 1 | ≥ 0.03 | ≥ 20 |
| Nanopore 10 kb Library | ≥ 40 | ≥ 2 | ≥ 20 |
| PacBio 8 kb Library | ≥ 50 | 10 μg/cell | ≥ 20 |
Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
Service Specifications
| Type of analysis | Recommended population scale | Sequencing strategy | Tag number |
|---|---|---|---|
| Genetic Maps | 2 parents and >150 offspring | Parents: 20x WGS Offspring: 10x |
Genome size: <400 Mb: WGS recommended <1 Gb: 100K tags 1-2 Gb: 200K tags >2 Gb: 300K tags Max 500K tags |
| Genome-Wide Association Studies (GWAS) | ≥ 200 samples | 10x | |
| Genetic Evolution | ≥ 30 samples, with ≥ 10 samples from each subgroup | 10x |
Service Requirements
- Concentration ≥ 5 ng/μL
- Total amount ≥ 80 ng
- Nanodrop OD260/280 = 1.6 – 2.5
- Agarose gel: No or limited degradation or contamination
Hi-C Based Genome Assembly
Service Specifications
| Library preparation | Sequencing strategy | Recommended data output | Quality control |
|---|---|---|---|
| Hi-C library | Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Sample Requirements
| Tissue | Required amount |
|---|---|
| Animal Viscera | ≥ 2 g |
| Animal Muscle | ≥ 2 g |
| Mammalian Blood | ≥ 2 mL |
| Poultry/Fish Blood | ≥ 2 mL |
| Plant - Fresh Leaf | ≥ 3 g |
| Cultured Cells | ≥ 1×107 |
| Insect | ≥ 2 g |
Genome-Wide Association Analysis (GWAS)
Service Specifications and Sample Requirements
| Type of sequencing | Recommended population scale | Sequencing strategy | Nucleotide requirements |
|---|---|---|---|
| Whole Genome Sequencing | 200 samples | 10x |
Concentration ≥ 1 ng/μL Total amount ≥ 30 ng Limited or no degradation or contamination |
| Specific-Locus Amplified Fragment (SLAF) |
Tag depth: 10x Number of tags: < 400 Mb: WGS recommended < 1 Gb: 100K tags 1-2 Gb: 200K tags > 2 Gb: 300K tags Max 500K tags |
Concentration ≥ 5 ng/μL Total amount ≥ 80 ng Nanodrop OD260/280 = 1.6–2.5 Agarose gel: no or limited degradation or contamination |
Evolutionary Genetics
Service Specifications and Sample Requirements
| Type of sequencing | Recommended population scale | Sequencing strategy | Nucleotide requirements |
|---|---|---|---|
| Whole Genome Sequencing | ≥ 30 individuals, with ≥ 10 individuals from each subgroup | 10x |
Concentration ≥ 1 ng/μL Total amount ≥ 30 ng Limited or no degradation or contamination |
| Specific-Locus Amplified Fragment (SLAF) |
Tag depth: 10x Number of tags: < 400 Mb: WGS recommended < 1 Gb: 100K tags 1-2 Gb: 200K tags > 2 Gb: 300K tags Max 500K tags |
Concentration ≥ 5 ng/μL Total amount ≥ 80 ng Nanodrop OD260/280 = 1.6–2.5 Agarose gel: no or limited degradation or contamination |
Comparative Genomics
Service Specifications
| Estimated turn-around time | Number of species | Analyses |
|---|---|---|
| 30 working days | 6 - 12 |
Gene family clustering Gene family expansion and contraction Phylogenetic tree construction Divergence time estimation (Fossil calibration required) LTR insertion time (For plants) Whole genome duplication (For plants) Selective pressure Synteny analysis |
Sample Requirements and Delivery
| Species | Tissue | Survey | PacBio CCS |
|---|---|---|---|
| Animal | Visceral tissue | 0.5 ~ 1 g | ≥ 3.5 g |
| Muscle tissue | ≥ 5.0 g | ||
| Mammalian blood | ≥ 5.0 mL | ||
| Poultry/Fish blood | - | ≥ 0.5 mL | |
| Plant | Fresh Leaf | 1 ~ 2 g | ≥ 5.0 g |
| Petal/Stem | 1 ~ 2 g | ≥ 10.0 g | |
| Root/Seed | 1 ~ 2 g | ≥ 20.0 g | |
| Cells | Cultured cell | - | ≥ 1 × 108 |
Prokaryotic RNA Sequencing
Service Specification and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| rRNA depleted directional library | Illumina PE150 | 1-2 Gb | Q30≥85% |
Sample Requirement
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 50 | ≥ 1 | OD260/280=1.8-2.0 OD260/230=1.0-2.5 Limited or no protein or DNA contamination shown on gel. |
RIN≥6.5 |
Eukaryotic mRNA Sequencing
Service Specifications
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| Poly A enriched | Illumina PE150 DNBSEQ-T7 |
6-10 Gb | Q30≥85% |
Minimum Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity | |
|---|---|---|---|---|
| Standard Library | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0 For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Directional Library | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0 For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1 g |
Insects: 6 g Crustacea: 300 mg |
1 tube | 106 cells |
Non-Reference Based mRNA Sequencing
Service Specifications
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| Poly A enriched | Illumina PE150 DNBSEQ-T7 |
6-10 Gb | Q30≥85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0 For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1 g |
Insects: 6 g Crustacea: 300 mg |
1 tube | 106 cells |
Full-Length mRNA Sequencing - Nanopore
Service Specifications
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| Poly A enriched | Nanopore PromethION 48 | 6/12 Gb | Average quality score: Q10 |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥7.0 For animals: RIN≥7.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1 g |
Insects: 6 g Crustacea: 300 mg |
1 tube | 106 cells |
Full-Length mRNA Sequencing - PacBio
Service Specifications
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| Poly A enriched mRNA CCS library | PacBio Sequel II PacBio Revio |
20/40 Gb 5/10 M CCS |
Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥7.5; For animals: RIN≥8.0; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1 g |
Insects: 6 g Crustacea: 300 mg |
1 tube | 106 cells |
PacBio 2+3 Full-Length mRNA Solution
Service Specifications
| Library | Sequencing strategy | Data recommended | Quality Control |
|---|---|---|---|
| Poly A enriched mRNA CCS library | PacBio Sequel II PacBio Revio |
20/40 Gb 5/10 M CCS |
Q30 ≥ 85% |
| Poly A enriched | Illumina PE150 | 6-10 Gb | Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity | |
|---|---|---|---|---|
| Illumina Library | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0 For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| PacBio Library | ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
Plants: RIN≥7.5 Animals: RIN≥8.0 5.0≥28S/18S≥1.0; limited or no baseline elevation |
Long Non-Coding RNA Sequencing - Illumina
Service Specifications
| Library | Platform | Data recommended | Quality Control |
|---|---|---|---|
| rRNA depleted directional Library | Illumina PE150 | 10-16 Gb | Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
RIN≥6.0 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 450 mg Viscera or Brain: 240 mg Muscle: 600 mg Bones, Hair or Skin: 1.5 g |
Insects: 9 g Crustacea: 450 mg |
2 tube | 106 cells |
Small RNA Sequencing - Illumina
Service Specifications
| Library | Platform | Data recommended | Quality Control |
|---|---|---|---|
| Size selected | Illumina SE150 | 10M-20M reads | Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
RIN≥6.0 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 450 mg Viscera or Brain: 240 mg Muscle: 600 mg Bones, Hair or Skin: 1.5 g |
Insects: 9 g Crustacea: 450 mg |
2 tube | 106 cells |
CircRNA Sequencing - Illumina
Service Specifications
| Library | Platform | Data recommended | Quality Control |
|---|---|---|---|
| rRNA depleted directional Library | Illumina PE150 | 16-20 Gb | Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
RIN≥6.0 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|---|---|---|---|---|
| Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
Heart or Intestine: 450 mg Viscera or Brain: 240 mg Muscle: 600 mg Bones, Hair or Skin: 1.5 g |
Insects: 9 g Crustacea: 450 mg |
2 tube | 106 cells |
Exosomal Small RNA Sequencing - Illumina
Service Specifications
| Library | Platform | Recommended data | Data QC |
|---|---|---|---|
| Exosomal miRNA isolation | Illumina SE50 | 10M-20M reads | Q30 ≥ 85% |
Sample Requirement
| Amount (ng) | Volume (μL) |
|---|---|
| 40 | 20 |
|
Exosome: 100 μL; 40 μg; 109 particles/mL Serum/Plasma: 5 mL, Cell culture supernatant: 100 mL, Urine: 100 mL, Saliva: 15 mL, Nasal discharge: 15 mL, Cerebrospinal: 15 mL, Amniotic fluid: 50 mL, Semen: 5 mL Bile: 5 mL, Ascites: 50 mL, Milk: 15 mL, Follicular fluid: 15 mL, Fecal supernatant: 50 mL, Microbial culture supernatant: 15 mL Animal tissue: 3 g, Plant tissue: 1 g |
|
Exosomal mRNA/LncRNA/CircRNA Sequencing - Illumina
Service Specifications
| Library | Platform | Recommended data | Data QC |
|---|---|---|---|
| Exosomal miRNA isolation | Illumina PE150 | 10-16 Gb | Q30 ≥ 85% |
Sample Requirement
| Amount (ng) | Volume (μL) |
|---|---|
| 40 | 20 |
|
Exosome: 100 μL; 40 μg; 109 particles/mL Serum/Plasma: 5 mL, Cell culture supernatant: 100 mL, Urine: 100 mL, Saliva: 15 mL, Nasal discharge: 15 mL, Cerebrospinal: 15 mL, Amniotic fluid: 50 mL, Semen: 5 mL Bile: 5 mL, Ascites: 50 mL, Milk: 15 mL, Follicular fluid: 15 mL, Fecal supernatant: 50 mL, Microbial culture supernatant: 15 mL Animal tissue: 3 g, Plant tissue: 1 g |
|
Metatranscriptome Sequencing
Service Specifications
| Sequencing platform | Sequencing Strategy | Data recommended | Data Quality Control |
|---|---|---|---|
| Illumina NovaSeq | PE150 | 12 Gb | Q30 ≥ 85% |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Volume (μL) | OD260/280 | OD260/230 | RIN |
|---|---|---|---|---|---|
| ≥ 50 | ≥ 1.0 | ≥ 20 | 1.8-2.0 | 1.0-2.5 | ≥ 6.5 |
Whole Transcriptome Sequencing - Illumina
Service Specifications
| Library | Sequencing Strategy | Data recommended | Quality Control |
|---|---|---|---|
| rRNA depleted | Illumina PE150 | 16-20 Gb | Q30 ≥ 85% |
| Size selected | Illumina SE50 | 10-20M reads | Q30 ≥ 85% |
Sample Requirements
| Conc. (ng/μl) | Amount (μg) | Purity | Integrity |
|---|---|---|---|
| ≥ 80 | ≥ 1.6 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
RIN ≥ 6.0 5.0 ≥ 28S/18S ≥ 1.0; limited or no baseline elevation |
Spatial Transcriptome
1. BMKMANU S3000 Spatial Transcriptome
| Sample Requirements | Library | Sequencing Strategy | Data Recommended | Quality Control |
|---|---|---|---|---|
| OCT-embedded cryo samples (approx. 6×6×6 mm³) 2 blocks per sample (1 for experiment, 1 for backup) |
S3000 cDNA Library | Illumina PE150 | 160K PE reads per 100uM (250 Gb) | RIN > 7 |
2. 10x Genomics Visium Spatial Transcriptome
| Sample Requirements | Library | Sequencing Strategy | Data Recommended | Quality Control |
|---|---|---|---|---|
| OCT-embedded cryo samples (approx. 6×6×6 mm³) 2 blocks per sample |
10X Visium cDNA Library | Illumina PE150 | 50K PE reads per spot (60 Gb) | RIN > 7 |
Single-Nuclei RNA Sequencing
Samples eligible for single nuclei RNA sequencing
| Cell / Tissue | Reason |
|---|---|
| Unfresh frozen tissue | Unable to get fresh or long-saved organizations |
| Muscle cell, Megakaryocyte, Fat... | Cell diameter is too large to enter the instrument |
| Liver... | Too fragile to break, unable to distinguish single cells |
| Neuron cell, Brain... | More sensitive, easy to stress, will change the sequencing results |
| Pancreas, Thyroid... | Rich in endogenous enzymes, affecting the production of single cell suspension |
Single-nucleus vs Single-cell
| Single-nucleus | Single-cell |
|---|---|
| Unlimited cell diameter | Cell diameter: 10-40 μm |
| The material can be frozen tissue | The material must be fresh tissue |
| Low stress of frozen cells | Enzyme treatment may cause cell stress reaction |
| No red blood cells need to be removed | Red blood cells need to be removed |
| Nuclear expresses bioinformation | The whole cell expresses bioinformation |
Service Specifications
| Sample Requirements | Library | Sequencing Strategy | Data Recommended | Quality Control |
|---|---|---|---|---|
| Animal tissue ≥ 200 mg Plant tissue ≥ 400 mg |
10x Genomics sn cDNA library | Illumina PE150 | 100K PE reads per cell (100-200 Gb) | 700-1200 nuclei/μl and nuclei integrity observed under microscope |
Hi-C Based Chromatin Interaction
Service Specifications
| Library | Sequencing Strategy | Recommended data output | Hi-C Signal Resolution |
|---|---|---|---|
| Hi-C library | Illumina PE150 | Chromatin loop: 150x TAD: 50x |
Chromatin Loop: 10Kb TAD: 40Kb |
Sample Requirements
| Sample type | Required amount |
|---|---|
| Animal tissue | ≥ 2g |
| Whole Blood | ≥ 2mL |
| Fungi | ≥ 1g |
| Plant - young tissue | 1g/aliquot, 2-4 aliquotes recommended |
| Cultured Cells | ≥ 1×107 |
ATAC-Seq
Service Specifications
| Library | Sequencing Strategy | Recommended data output | Quality control |
|---|---|---|---|
| ATAC-seq | Illumina PE150 | > 20M reads Depending on genome size (Human: 50M reads) |
Q30 ≥ 85% |
Sample Requirements
| Sample | Requirement |
|---|---|
| Cell number | ≥ 106 cells |
| Tissue weight | ≥ 200 mg fresh tissue |
| Blood | ≥ 4 mL |
Whole Genome Bisulfate Sequencing (WGBS)
Service Specifications
| Library | Sequencing Strategy | Recommended data output | Quality control |
|---|---|---|---|
| Bisulfite treated | Illumina PE150 | 30x depth | Q30 ≥ 85% Bisulfite conversion > 99% |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Additional requirements |
|---|---|---|
| ≥ 5 | ≥ 400 ng | Limited degradation or contamination |
Reduced Representation Bisulfate Sequencing (RRBS)
Service Specifications
| Library | Sequencing Strategy | Recommended data output | Quality control |
|---|---|---|---|
| MspI digested and Bisulfite treated library | Illumina PE150 | 8 Gb | Q30 ≥ 85% Bisulfite conversion > 99% MspI cutting efficiency > 95% |
Sample Requirements
| Concentration (ng/μL) | Total amount (μg) | Additional requirements |
|---|---|---|
| ≥ 30 | ≥ 1 | Limited degradation or contamination |
Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
Service Specifications
| Library | Sequencing Strategy | Recommended data output | Quality control |
|---|---|---|---|
| Purified DNA after Immunoprecipitation | Illumina PE150 | 10 Gb | Q30 ≥ 85% |
Sample Requirements
- Total amount: ≥ 10 ng
- Fragment size distribution: 100-750 bps
Label-free DIA Quantitation
Sample Requirements
| Sample Type | Label-free DIA/DDA | |
|---|---|---|
| Animal | Common animal tissues: animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, barin, etc | ≥ 5 mg |
| Hard Tissue (Cartilage, Bone) | ≥ 100 mg | |
| White Adipose tissue | ≥ 100 mg | |
| Cell | Suspended cells, adherent cells | ≥ 5 × 10⁶ cells |
| Cell culture supernatant | ≥ 5 mL | |
| Fluid | Plasma, Serum | ≥ 100 μL |
| Saliva, Breast milk, Prostatic fluid, Rumen fluid | ≥ 100 μL | |
| Urine/Ascites/Pleural effusion/Lavage fluid, etc | ≥ 15 mL | |
| Tear fluid | ≥ 15 μL | |
| Tear fluid filter strip | ≥ 2 strips | |
| Aqueous humor | ≥ 100 μL | |
| Plant | Plant buds, pollen | ≥ 100 mg |
| Plant seeds and fruits (low moisture, rice/wheat seeds) | ≥ 400 mg | |
| Plant seeds and fruits (high moisture, apple, peach, pear) | ≥ 500 mg | |
| Tender plant shoots (leaf buds, young leaves), algae | ≥ 200 mg | |
| Mature leaves, roots, stems and bark | ≥ 300 mg | |
| Other | Feces, Intestinal contents | ≥ 50 mg |
| Distiller’s yeast, fermenting grains, Da Qu fermentation | ≥ 500 mg | |
| Microorganism | Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast) | Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells |
| Protein Solution | Complex protein solution, protein powder | ≥ 40 μg, Conc.: ≥ 0.5 μg/μL |
Isobaric Label (TMT: Tandem Mass Tags)
Sample Requirements
| Sample Type | Label-free DIA/DDA | |
|---|---|---|
| Animal | Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc) | ≥ 10 mg |
| Hard Tissue (Cartilage, Bone) | ≥ 100 mg | |
| White Adipose tissue | ≥ 100 mg | |
| Cell | Suspended cells, adherent cells | ≥ 5 × 10⁶ cells |
| Cell culture supernatant | ≥ 10 mL | |
| Fluid | Plasma, Serum | ≥ 200 μL |
| Saliva, Breast milk, Prostatic fluid, Rumen fluid | ≥ 200 μL | |
| Urine/Ascites/Pleural effusion/Lavage fluid, etc | ≥ 30 mL | |
| Tear fluid | ≥ 25 μL | |
| Tear fluid filter strip | ≥ 2 strips | |
| Aqueous humor | ≥ 300 μL | |
| Plant | Plant buds, pollen | ≥ 100 mg |
| Plant seeds and fruits (low moisture, rice/wheat seeds) | ≥ 500 mg | |
| Plant seeds and fruits (high moisture, apple, peach, pear) | ≥ 1 g | |
| Tender plant shoots (leaf buds, young leaves), algae | ≥ 300 mg | |
| Mature leaves, roots, stems and bark | ≥ 500 mg | |
| Other | Feces, Intestinal contents | ≥ 100 mg |
| Distiller’s yeast, fermenting grains, Da Qu fermentation | ≥ 1 g | |
| Microorganism | Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast) | Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells |
| Protein Solution | Complex protein solution, protein powder | ≥ 100 μg, Conc.: ≥ 0.5 μg/μL |
Label-free DDA Proteomics
Sample Requirements
| Sample Type | Label-free DIA/DDA | |
|---|---|---|
| Animal | Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc) | ≥ 5 mg |
| Hard Tissue (Cartilage, Bone) | ≥ 100 mg | |
| White Adipose tissue | ≥ 100 mg | |
| Cell | Suspended cells, adherent cells | ≥ 5 × 10⁶ cells |
| Cell culture supernatant | ≥ 5 mL | |
| Fluid | Plasma, Serum | ≥ 100 μL |
| Saliva, Breast milk, Prostatic fluid, Rumen fluid | ≥ 100 μL | |
| Urine/Ascites/Pleural effusion/Lavage fluid, etc | ≥ 15 mL | |
| Tear fluid | ≥ 15 μL | |
| Tear fluid filter strip | ≥ 2 strips | |
| Aqueous humor | ≥ 100 μL | |
| Plant | Plant buds, pollen | ≥ 100 mg |
| Plant seeds and fruits (low moisture, rice/wheat seeds) | ≥ 400 mg | |
| Plant seeds and fruits (high moisture, apple, peach, pear) | ≥ 500 mg | |
| Tender plant shoots (leaf buds, young leaves), algae | ≥ 200 mg | |
| Mature leaves, roots, stems and bark | ≥ 300 mg | |
| Other | Feces, Intestinal contents | ≥ 50 mg |
| Distiller’s yeast, fermenting grains, Da Qu fermentation | ≥ 500 mg | |
| Microorganism | Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast) | Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells |
| Protein Solution | Complex protein solution, protein powder | ≥ 40 μg, Conc.: ≥ 0.5 μg/μL |
Untargeted Metabolomics
Sample Requirements
| Sample Type | Recommend | Minimum |
|---|---|---|
| Serum, Plasma, Urine, Other body fluids (Amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.) | ≥ 10 mg | ≥ 1 mg |
| Filter paper strips, Swabs | ≥ 4 strips | ≥ 2 strips |
| Exosomes (isolated exosomes by clients) | ≥ 500 μL, ~1011 particles/mL | ≥ 300 μL, ~108 particles/mL |
| Semen plasma, Amniotic fluid, Prostatic fluid, Rumen fluid, Exhaled breath condensate, Gastric lavage fluid, Bronchoalveolar lavage fluid, Sweat, Saliva, Sputum, Milk, Cell culture supernatant, Fermentation broth | ≥ 500 μL | ≥ 100 μL |
| Animal & Clinical tissues, Feces, Intestinal contents, Microbial organisms, Spinal cord, Cartilage | ≥ 100 mg | ≥ 25 mg |
| Cell | 1 × 10? cells | 5 × 10? cells |
| Distiller's yeast, Fermenting grains, Da Qu fermentation | ≥ 500 mg | ≥ 100 mg |
| Soil, Sludge, Marine sediment | ≥ 500 mg | ≥ 100 mg |
Lipidomics Profiling
Sample Requirements
| Sample Type | Recommend | Minimum |
|---|---|---|
| Serum, Plasma, Urine, Other body fluids (Amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.) | ≥ 10 mg | ≥ 1 mg |
| Filter paper strips, Swabs | ≥ 4 strips | ≥ 2 strips |
| Exosomes (isolated exosomes by clients) | ≥ 500 μL, ~1011 particles/mL | ≥ 300 μL, ~108 particles/mL |
| Semen plasma, Amniotic fluid, Prostatic fluid, Rumen fluid, Exhaled breath condensate, Gastric lavage fluid, Bronchoalveolar lavage fluid, Sweat, Saliva, Sputum, Milk, Cell culture supernatant, Fermentation broth | ≥ 500 μL | ≥ 100 μL |
| Animal & Clinical tissues, Feces, Intestinal contents, Microbial organisms, Spinal cord, Cartilage | ≥ 100 mg | ≥ 25 mg |
| Cell | 107 | 5 * 106 |
| (Each sample must have the same number of cells) | ||
| Distiller's yeast, Fermenting grains, Da Qu fermentation | ≥ 500 mg | ≥ 100 mg |
| Soil, Sludge, Marine sediment | ≥ 500 mg | ≥ 100 mg |
| Plant - Fresh plant tissue | ≥ 500 mg | ≥ 100 mg |
| Plant - Freezed-dried plant tissue | ≥ 500 mg | ≥ 150 mg |
| Plant - Traditional Chinese medicine soup | Freeze-dried powder ≥ 200 mg, Medicine soup ≥ 1 mL | Freeze-dried powder ≥ 25 mg, Medicine soup ≥ 100 μL |
| Plant - Chinese medicine pills | ≥ 1 g | ≥ 25 mg |
Targeted Metabolomics
Sample Requirements
| Sample Type | Recommend | Minimum |
|---|---|---|
| Serum, Plasma, Urine | ≥ 200 μL | ≥ 100 μL |
| Animal & Clinical tissues, Feces, Intestinal contents, Microorganisms | ≥ 100 mg | ≥ 40 mg |
| Cell | 2 × 107 cells | 2 × 105 cells |
| Cell culture medium, Fermental medium | ≥ 1 mL | ≥ 200 μL |
Biological Replicates Requirements
| Sample Type | Recommend Biological Duplicates | Minimum Biological Duplicates |
|---|---|---|
| Cell and Microorganism | ≥ 6 ea | ≥ 3 ea |
| Animal | ≥ 10 ea | ≥ 6 ea |
| Human | ≥ 30 ea | ≥ 10 ea |