Hi-C Based Chromatin Interaction

Service Specifications

Library	Sequencing Strategy	Recommended data output	Hi-C Signal Resolution
Hi-C library	Illumina PE150	Chromatin loop: 150x TAD: 50x	Chromatin Loop: 10Kb TAD: 40Kb

Sample Requirements

Sample type	Required amount
Animal tissue	≥ 2g
Whole Blood	≥ 2mL
Fungi	≥ 1g
Plant - young tissue	1g/aliquot, 2-4 aliquotes recommended
Cultured Cells	≥ 1×107

ATAC-Seq

Service Specifications

Library	Sequencing Strategy	Recommended data output	Quality control
ATAC-seq	Illumina PE150	> 20M reads Depending on genome size (Human: 50M reads)	Q30 ≥ 85%

Sample Requirements

Sample	Requirement
Cell number	≥ 106 cells
Tissue weight	≥ 200 mg fresh tissue
Blood	≥ 4 mL

Whole Genome Bisulfate Sequencing (WGBS)

Service Specifications

Library	Sequencing Strategy	Recommended data output	Quality control
Bisulfite treated	Illumina PE150	30x depth	Q30 ≥ 85% Bisulfite conversion > 99%

Sample Requirements

Concentration (ng/μL)	Total amount (μg)	Additional requirements
≥ 5	≥ 400 ng	Limited degradation or contamination

Reduced Representation Bisulfate Sequencing (RRBS)

Service Specifications

Library	Sequencing Strategy	Recommended data output	Quality control
MspI digested and Bisulfite treated library	Illumina PE150	8 Gb	Q30 ≥ 85% Bisulfite conversion > 99% MspI cutting efficiency > 95%

Sample Requirements

Concentration (ng/μL)	Total amount (μg)	Additional requirements
≥ 30	≥ 1	Limited degradation or contamination

Chromatin Immunoprecipitation Sequencing (ChIP-Seq)

Service Specifications

Library	Sequencing Strategy	Recommended data output	Quality control
Purified DNA after Immunoprecipitation	Illumina PE150	10 Gb	Q30 ≥ 85%

Sample Requirements

• Total amount: ≥ 10 ng
• Fragment size distribution: 100-750 bps