Label-free DIA Quantitation

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues: animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, barin, etc	≥ 5 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 5 mL
Fluid	Plasma, Serum	≥ 100 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 100 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 15 mL
	Tear fluid	≥ 15 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 100 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 400 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 500 mg
	Tender plant shoots (leaf buds, young leaves), algae	≥ 200 mg
	Mature leaves, roots, stems and bark	≥ 300 mg
Other	Feces, Intestinal contents	≥ 50 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 500 mg
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 40 μg, Conc.: ≥ 0.5 μg/μL

Isobaric Label (TMT: Tandem Mass Tags)

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc)	≥ 10 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 10 mL
Fluid	Plasma, Serum	≥ 200 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 200 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 30 mL
	Tear fluid	≥ 25 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 300 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 500 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 1 g
	Tender plant shoots (leaf buds, young leaves), algae	≥ 300 mg
	Mature leaves, roots, stems and bark	≥ 500 mg
Other	Feces, Intestinal contents	≥ 100 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 1 g
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 100 μg, Conc.: ≥ 0.5 μg/μL

Label-free DDA Proteomics

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc)	≥ 5 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 5 mL
Fluid	Plasma, Serum	≥ 100 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 100 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 15 mL
	Tear fluid	≥ 15 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 100 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 400 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 500 mg
	Tender plant shoots (leaf buds, young leaves), algae	≥ 200 mg
	Mature leaves, roots, stems and bark	≥ 300 mg
Other	Feces, Intestinal contents	≥ 50 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 500 mg
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 40 μg, Conc.: ≥ 0.5 μg/μL