Label-free DIA Quantitation

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues: animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, barin, etc	≥ 5 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 5 mL
Fluid	Plasma, Serum	≥ 100 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 100 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 15 mL
	Tear fluid	≥ 15 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 100 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 400 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 500 mg
	Tender plant shoots (leaf buds, young leaves), algae	≥ 200 mg
	Mature leaves, roots, stems and bark	≥ 300 mg
Other	Feces, Intestinal contents	≥ 50 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 500 mg
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 40 μg, Conc.: ≥ 0.5 μg/μL

Isobaric Label (TMT: Tandem Mass Tags)

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc)	≥ 10 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 10 mL
Fluid	Plasma, Serum	≥ 200 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 200 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 30 mL
	Tear fluid	≥ 25 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 300 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 500 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 1 g
	Tender plant shoots (leaf buds, young leaves), algae	≥ 300 mg
	Mature leaves, roots, stems and bark	≥ 500 mg
Other	Feces, Intestinal contents	≥ 100 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 1 g
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 100 μg, Conc.: ≥ 0.5 μg/μL

Label-free DDA Proteomics

Sample Requirements

Sample Type		Label-free DIA/DDA
Animal	Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc)	≥ 5 mg
	Hard Tissue (Cartilage, Bone)	≥ 100 mg
	White Adipose tissue	≥ 100 mg
Cell	Suspended cells, adherent cells	≥ 5 × 10⁶ cells
	Cell culture supernatant	≥ 5 mL
Fluid	Plasma, Serum	≥ 100 μL
	Saliva, Breast milk, Prostatic fluid, Rumen fluid	≥ 100 μL
	Urine/Ascites/Pleural effusion/Lavage fluid, etc	≥ 15 mL
	Tear fluid	≥ 15 μL
	Tear fluid filter strip	≥ 2 strips
	Aqueous humor	≥ 100 μL
Plant	Plant buds, pollen	≥ 100 mg
	Plant seeds and fruits (low moisture, rice/wheat seeds)	≥ 400 mg
	Plant seeds and fruits (high moisture, apple, peach, pear)	≥ 500 mg
	Tender plant shoots (leaf buds, young leaves), algae	≥ 200 mg
	Mature leaves, roots, stems and bark	≥ 300 mg
Other	Feces, Intestinal contents	≥ 50 mg
	Distiller’s yeast, fermenting grains, Da Qu fermentation	≥ 500 mg
Microorganism	Prokaryotic bacteria (E. coli, Weissella spp.), fungi (yeast)	Biomass: ≥ 50 mg or Cell count: ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 40 μg, Conc.: ≥ 0.5 μg/μL

Targeted Proteomics (PRM: Parallel Reaction Monitoring)

Sample Requirements

Sample Type	Recommend	Minimum
Animal	Common animal tissues (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc)	≥ 10 mg	≥ 1 mg
	Mollusks (Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)	≥ 10 mg	≥ 2 mg
Cell	Suspended cells, adherent cells	≥ 1 × 10⁷ cells	≥ 1 × 10⁶ cells
	Cell culture supernatant	≥ 10 mL
Exosome	Exosome isolated by customer	≥ 50 μg, ≥ 0.5 μg/μL
Fluid	Plasma, Serum (remove highly-abundant protein)	≥ 200 μL
	Plasma, Serum (with highly-abundant protein)	≥ 10 μL	≥ 3 μL
	Amniotic fluid, cerebrospinal fluid, semen, etc. (remove highly-abundant protein)	≥ 1 mL	≥ 500 μL
	Amniotic fluid, cerebrospinal fluid, semen, etc. (with highly-abundant protein)	≥ 200 μL	≥ 100 μL
	Saliva, Milk	≥ 200 μL	≥ 100 μL
	Urine	≥ 30 mL	≥ 15 mL
	Tear	≥ 15 μL	≥ 10 μL
Plant	Twigs of plants (leaf buds, tender leaves), algae	≥ 300 mg	≥ 200 mg
	Old leaves, roots, stems, bark of plants	≥ 1 g	≥ 500 mg
	Plant buds, pollen	≥ 100 mg	≥ 50 mg
	Plant seeds (rice/wheat seeds, etc.), fruits (apples, peaches, pears)	≥ 1 g	≥ 500 mg
	Plant seeds (rice/wheat seeds, etc.)	≥ 300 mg	≥ 100 mg
Other	Feces	≥ 300 mg
Microorganism	Prokaryotic bacteria (e.g. E. coli, Staphylococcus aureus, etc.), fungi (e.g. yeast, etc.)	Thallus ≥ 50 mg, cells ≥ 5 × 10⁶ cells
Protein Solution	Complex protein solution, protein powder	≥ 100 μg, ≥ 0.5 μg/μL	≥ 50 μg, ≥ 0.5 μg/μL